علوم زیستی دریا
Mehrdad Sarkheil; gholamreza rafiee; mehrdad farhangi
Abstract
In this study, effects of four concentrations of waterborne calcium (8, 25, 50, 100 mg/l) on eyed egg percentage, hatching percentage and ions (Ca, K, Cu and Mn) uptake of rainbow trout eggs in a water recirculating system were determined. Twelve experimental units, with the capacity of holding 45 liter ...
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In this study, effects of four concentrations of waterborne calcium (8, 25, 50, 100 mg/l) on eyed egg percentage, hatching percentage and ions (Ca, K, Cu and Mn) uptake of rainbow trout eggs in a water recirculating system were determined. Twelve experimental units, with the capacity of holding 45 liter of water were used and 21g egg introduced in each one. Different concentrations of ions (Ca,Na,K,Mg,P,Fe,Cu,Zn,Mn) in treatments were prepared by adding analytical salts (Merck) into double-de-ionized distilled water. Dissolved oxygen and water temperature variations during incubation were 9.80-11.11mg/l and 7.5-9 ºC respectively. The eyed egg percentage was not significantly (P>0.05) different among treatments. The hatching percentage of eggs were significantly (P
Ahmad Imani; Mehrdad Farhangi; Gholamreza Rafiee; Razieh Yazdanparast; Isabel Vasquez Baanante
Volume 11, Issue 3 , November 2012, , Pages 4-15
Abstract
We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by ...
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We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by proteinase-K digestion, phenol: chloroform: isoamyl alcohol extraction and ethanol precipitation. The quality and quantity of DNA was assessed using 1% agarose gel electrophoresis and spectrophotomeric method. Polymerase chain reactions (PCR) were conducted on the target DNA using 6 primers RAPD. PCR products were separated by electrophoresis on polyacrylamid gels (8%) that were stained using silver nitrate. Allele frequency, the effective and real allele, expected and observed heterozygosis, genetic diversity, Shannon Information index, Gst and gene based on analysis of molecular variance (AMOVA) were calculated using the GENALEX and Pop Gene software. The average of Shannon index for Hendijan, Khore zangi and Delvar was respectively 0.3887, 0.2860 and 0.3509. Highest of Gst observed between Hendijan and Khore zangi (0.260) and lowest between Khore zangi and Delvar (0.195). Highest of gene flow (Nm) was observed between Delvar and Khore zangi (1.033) and lowest between Hendijan and Khore zangi (0.687). Highest of Genetic identity observed between Delvar and Khore zangi (0.924) but lowest was between Hendijan and Khore zangi community (0.833). Eventually this study show that three community of our research are separate and belong to two clusters.